Figure 4From: Toll-Like Receptor 4 Engagement Inhibits Adenosine 5′-Monophosphate-Activated Protein Kinase Activation through a High Mobility Group Box 1 Protein-Dependent MechanismDiminished expression of HMGB1 increases AMPK activity. (A, B) RAW 264.7 cells were treated with HMGB1-specific siRNA or control scrambled siRNA and then incubated with or without AICAR (1 mmol/L) for 1 h. Representative Western blots (A) and bend optical densitometry (B) show the levels of phosphorylated AMPK and ACC, as well as total AMPK, ACC and HMGB1 (means ± SD, n = 3, *P < 0.05, **P < 0.01). ns, Nonsignificant.Back to article page