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Figure 6 | Molecular Medicine

Figure 6

From: Effect of Nuclear Factor κB Inhibition on Serotype 9 Adeno-Associated Viral (AAV9) Minidystrophin Gene Transfer to the mdx Mouse

Figure 6

Quantification of vector DNA and mRNA transcripts in quadriceps. DNA and RNA were isolated from quadriceps muscle tissues from AAV + NBD peptide- and AAV + saline-treated mdx mice and analyzed by real-time qPCR. Vector DNA levels were quantified by primers/probes specific for AAV9 minidystrophin DNA, using isolated AAV9 minidystrophin vector DNA to generate a standard curve. The numbers of nuclei per sample were quantified by real-time qPCR using primers/probes specific for the ApoB gene. (A) Vector copy numbers were normalized to nuclei copy number to yield vector genomes/nucleus. Isolated muscle mRNA was reverse-transcribed, and cDNA samples were analyzed by real-time qPCR with primers/probe specific for minidystrophin cDNA to assess levels of vector mRNA and also with primers/probe specific for GAPDH. (B) Vector mRNA expression was normalized to GAPDH. Data are shown as mean ± SEM; *significant difference from AAV + saline-treated mdx mice (P < 0.05); n = number of animals analyzed.

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