Figure 2
From: The Vitamin D Receptor Regulates Rheumatoid Arthritis Synovial Fibroblast Invasion and Morphology
Quantitative polymerase chain reaction (qPCR) analyses of MMP-1 and VDR in FLSs. Calcitriol (CALC) suppresses IL-1β-induced expression of MMP-1 in FLS from DA (50 nmol/L) by 95% (A) and in FLS from RA (1 µmol/L) by 73.5% (B). Calcitriol treatment for 24 h increased VDR mRNA levels by 2.6-fold in DA FLSs cultured on polystyrene plates (50 nmol/L; n = 7 per group; Figure 2C) (C) and by sixfold in RA FLSs cultured on Matrigel compared with DMSO control (1 µmol/L; n = 5 per group) (D).