masTF binds β3 integrins on murine EC and elicits β3 integrin-dependent cell migration. (A) bEnd.3 cells avidly adhere to masTF, mflTF and hasTF (n µ 6). (B) bEnd.3 cells were pre-incubated with integrin-blocking antibodies and seeded onto BSA- or masTF-coated wells (n ≥ 3). Flattened cells were counted. (C) Transwell inserts were coated with BSA (negative control), 1% gelatin (positive control) and 50 µg/mL masTF. Cells were fixed with 4% formaldehyde and stained with 0.1% crystal violet; quantifications are shown in the graph; representative images are on the left (n ≥ 3). *p < 0.01.