Figure 5

masTF and hasTF elicit analogous changes in the gene expression profile of murine ECs. (A) Cellular distribution of p65/RelA in bEnd.3 cells stimulated with masTF and hasTF (n ≥ 3). White arrows indicate nuclear localization. (B) Heat map, gene cluster analysis of bEnd.3 cells exposed to 50 nmol/L of masTF and 50 nmol/L of hasTF for 4 h; 50% glycerol/PBS served as vehicle control. The heat map comprises the genes for which expression was either significantly upregulated by at least 1.2-fold or downregulated by at least 0.9-fold. Results shown are representative of three independent experiments; red denotes upregulation and green denotes downregulation.