Figure 2
From: Ymer Acts as a Multifunctional Regulator in Nuclear Factor-κB and Fas Signaling Pathways
Resistance to septic shock in Ymer Tg mice. (A) Immunoblot analysis of Ymer Tg mice treated with TNFα or LPS. Three days after intraperitoneal injection with TNFα or LPS, cell lysates from the livers of WT and Ymer Tg mice were used for immunoblot analysis with Abs against PARP,RIP,IKK and P-IKK. (B) Survival study of Ymer Tg mice by LPS. WTand Ymer Tg mice were injected with LPS (50 mg/kg) and then observed for 24 h (WT, n = 4; Tg413, n = 4; Tg545, n = 5). (C) Production of inflammatory cytokines in Ymer Tg mice. Expression levels of IL-1β, IL-6, TNFα, IFNα and iNOS were measured by qRT-PCR at 4 h after LPS injection. (D) Secretion of IL-6 in sera of Ymer Tg mice. Four hours after LPS injection, IL-6 concentrations of sera from WTand Ymer Tg mice were measured by ELISA (WT, n = 3; Tg413, n = 4; Tg545, n = 5).