Figure 5

Acceleration of Fas-induced apoptosis in livers of Ymer Tg mice. (A) Survival study for Fas-induced cell death was performed with anti-Fas Ab (10 ng/mouse) intraperitoneal injection into WT and Ymer Tg mice (WT, n = 5; Tg⁴¹³, n = 6; Tg⁵⁴⁵, n = 6). Survival of the mice was observed for 24 h after injection. Statistical analysis was performed by using the Kaplan-Meier test. (B) Livers treated with anti-Fas Ab were dissected out at 6 h after injection. (C) Livers treated with anti-Fas Ab were dissected out at 4 h after injection and used for histological analysis with HE staining. Scale bars indicate 100 nm in low magnification and 20 nm in high magnification (boxed). (D) Immunoblot analysis of apoptosis-related proteins in anti-Fas Ab-treated livers from Ymer Tg mice. Two hours after injection with anti-Fas Ab, cell lysates from livers were probed with the indicated Abs.