Figure 5

Effect of axitinib on the expression of ABCG2in MDR cells at the protein (A) and mRNA (B, C) levels. S1-M1-80 cells were treated with axitinib at 1.0 µmol/L for 12, 24 and 48 h, or at 0.25, 0.5, 1.0 and 2.0 µmol/L for 48 h, respectively. The change at mRNA level was determined by reverse transcription-PCR (B) and quantitative real-time PCR (C) as described in Materials and Methods. Independent experiments were performed at least three times and results from a representative experiment are shown.