Figure 5

Expression of cleaved PARP-1 and PARP-1 activity in the pancreas of WT mice before and after low-dose STZ treatment. (A) Levels of cleaved PARP-1 protein expression in the islets isolated from young and old mice by Western blot analysis. (B) Analysis of immunoblot of cleaved PARP-1 protein normalized to β-actin. Values were representative of islets from 4–5 mice per group. **p < 0.01 versus young WT mice. (C) The PARP-1 activity of pancreatic islets was determined with the universal colorimetric PARP-1 assay kit, and results were expressed as micromoles of p-nitrophenol formed per minute per milligram of PARP-1 protein. Values were representative of islets from 4–5 mice per group. **p < 0.01 versus young WT mice.