Blockade of TRPV1 activation abrogates evodiamine-increased AMPK and eNOS phosphorylation in ECs. BAECs were pretreated with or without 500 nmol/L EGTA (A), 10 µmol/L CPZ (B, C) or 5 µmol/L KN62 (D) for 1 h, then incubated with evodiamine (1 µmol/L) for 15 min. Western blot analysis of protein level of phosphorylated AMPK at Thr172, AMPK, phosphorylated eNOS at Ser635 and Ser1179, and eNOS. Data are mean ± SD from five independent experiments. *P < 0.05 versus vehicle treated group, #P < 0.05 versus evodiamine-treated group.