AMPK mediates evodiamine-induced eNOS phosphorylation, NO production and tube formation in ECs. (A) BAECs were pretreated or without 10 µmol/L compound C (C.C) for 1 h, then incubated with evodiamine (1 µmol/L) for 15 min. Cellular lysates were immunoblotted with phosphorylated AMPK at Thr172, AMPK, phosphorylated eNOS at Ser635 and Ser1179, and eNOS. (B) BAECs were pretreated or without compound C (10µmol/L) for 1 h, then incubated with evodiamine (1µmol/L) for 24 h. The level of nitrite in cultured medium was analyzed by Griess assay. (C) BAECs were cultured in precoated matrigel in the presence of the indicated treatments for 24 h. Tube formation was photographed, and bar graphs indicate the number of branch points in 5 randomly selected microscopy views. Data are mean ± SD from five independent experiments. *P < 0.05 versus vehicle-treated group, #P < 0.05 versus evodiamine-treated group.