Deletion of TRPV1 impairs evodiamine-induced phosphorylation of AMPK and eNOS. (A) MAECs were isolated from 8-wk-old male WT and TRPV1−/− mice and treated with 1 µmol/L evodiamine for 15 min. (B) Male WT mice were killed after intraperitoneal (ip) injection with evodiamine (3 mg/kg) for the indicated times. The control mice received the same amount of vehicle. (C) Male WT and TRPV1−/− mice were killed after evodiamine injection (3 mg/kg) for 4 h. Cellular or tissue extracts from aortas were immunoblotted with phosphorylated AMPK at Thr172, AMPK, phosphorylated eNOS at Ser635 and Ser1179, and eNOS. Data are mean ± SD from five mice. *P < 0.05 versus time zero or WT mice without evodiamine treatment, #P < 0.05 versus WT mice with evodiamine treatment.