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Figure 1 | Molecular Medicine

Figure 1

From: Mice with a D190N Mutation in the Gene Encoding Rhodopsin: A Model for Human Autosomal-Dominant Retinitis Pigmentosa

Figure 1

Germline transmission of D190N knock-in allele. (A) Genomic locus of rhodopsin (top panel); targeting vector (middle panel) and the resultant targeted D190N allele (bottom panel) are shown. The neomycin cassette was subsequently removed. 5′ and 3′ probes were used to confirm the integrity of the targeted locus. (B) Direct DNA sequencing from D190N/+ mutant mouse DNA showing heterozygous base-substitution at position 190 in D190N/+. Direct sequencing from the D190N/D190N mouse DBA revealed a homozygous base substitution at the 190 codon with a predicted missense mutation of D190N (GAC to AAC): GAC (Asp/D) aspartic acid to AAC (Asn/N) asparagine.

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Molecular Medicine

ISSN: 1528-3658