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Figure 5 | Molecular Medicine

Figure 5

From: Mice with a D190N Mutation in the Gene Encoding Rhodopsin: A Model for Human Autosomal-Dominant Retinitis Pigmentosa

Figure 5

Immunohistochemistry and immunoblotting shows rhodopsin expression in wt and D190N/+ retinas. Rhodopsin correctly localizes in the OS of wt retinas at P21 (A) and P210 (C). In D190N/+ mice, rhodopsin localizes correctly in the OS of the retina at P21 (B) and P210 (D). Compared to the wt controls, D190N/+ mice show shortened OS at both P21 and P210. DAPI was used to counterstain nuclei. GCL, ganglion cell layer; INL, inner nuclear layer. Representative immunoblot of retinal extract prepared from wt and D190N/+ mice demonstrates linearity in rhodopsin levels (E). Each lane represents the amount loaded (mL) per retina (200 mL total sample volume). Scale bar: 50 µm.

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