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Figure 4 | Molecular Medicine

Figure 4

From: Flexible Targeting of ErbB Dimers That Drive Tumorigenesis by Using Genetically Engineered T Cells

Figure 4

T1E28z engineered T cells destroy human head and neck cancer cells. (A) Twelve human SCCHN cell lines (and MDA-MB-435 control cells) were analyzed for cell surface expression of ErbB1–4 by FACS. Staining with the indicated ErbB-specific (open histogram) or isotype control (filled histogram) antibody is indicated. (B) Confluent monolayers of the indicated tumor cell lines (24-well plate, 1.9 cm2) were cocultivated with 1 × 106 T1E28z+ T cells. Control T cells expressed T1NA (a matched endodomain-truncated CAR) or were untransduced (Untrans.). After 72 h, residual monolayers were fixed and stained with crystal violet. Punctate staining in T1E28z wells represents adherent lymphocytes. (C) One million of the indicated T-cell populations were cocultivated overnight with a confluent 1.9-cm2 monolayer of luciferase (LUC)-expressing HN3 cells or Vβ6 cells. After the specified interval, T cells were removed and residual viable tumor cells were quantified using XTT (mean ± SD, n = 3). Percentage lysis was calculated as (100 — cell viability).

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