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Figure 9 | Molecular Medicine

Figure 9

From: Flexible Targeting of ErbB Dimers That Drive Tumorigenesis by Using Genetically Engineered T Cells

Figure 9

In vivo activity of T1E28z+ T cells against an ErbB2/3-expressing aggressive tumor xenograft. (A) MDA-MB-435 cells were engineered to coexpress MUC1, ErbB2 and firefly luciferase (MUC-LUC ERBB2). FACS plots show staining with ErbB-specific (open histograms) and isotype control antibodies (filled histograms). (B) MUC-LUC ErbB2 tumor cells were inoculated intraperitoneally in 20 SCID Beige mice. After 3 d, mice (n = 5 per group) were treated with PBS, IL-4 (125 ng × 3 doses per wk), 2 × 107 T4+ T cells or the combination of T4+ T cells + IL-4. Tumor status was monitored by bioluminescence imaging. Data shown are mean ± standard error of the mean of each group. (***P < 0.0001 comparing either T4 or T4 + IL-4 versus either IL-4 or PBS; **P < 0.0001 comparing either T4 or T4 + IL-4 against PBS). (C) Serial bioluminescence emission by mice in the T4 and T4 + IL-4 groups (mean ± SEM), represented on a semi-log plot (*P = 0.015 on d 35; two-way ANOVA).

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