Figure 3
Effect of MSC administration on the number of ubiquitin-immunoreactive (A), ChAT-immunoreactive (B) and GLT1-immunoreactive (C) cells and on mRNA expression of GLT1 (D) in the spinal cord of SOD1/G93A mice. SOD1/G93A mice were treated with 106 MSCs or saline at d 90 and sacrificed at d 125. The following antibodies were used for histology: mouse anti-ubiquitin, 1:250; rabbit anti-ChAT, 1:500; guinea pig anti-GLT1, 1:100. Seven MSC-treated and seven saline-injected SOD1/G93A mice were used. Quantification was performed on six sections per animal, from three segments apart. mRNA was detected by RT-PCR using GAPDH mRNA as a reference. Data (means ± SEM) represent the number of positive cells/mm2 (A-C) or fold induction with respect to controls (D). *p < 0.01 (Mann-Whitney).