Figure 4

Effects of MSC administration on IB4-positive microglia cells and GFAP-positive astrocytes. SOD1/G93A mice were treated with 10⁶ MSCs or saline at d 90 and sacrificed at d 125. Spinal cord (A, D), brain cortex (B, E) and hippocampus (C, F) 5-µm sections were stained for histological examination. Rabbit anti-cow GFAP diluted 1:100 (A–C), or isolectin IB4 conjugated antibody, diluted 1:100 (D–F), was used. Seven MSC-treated and seven saline-injected mice were used for the experiments. Quantification was performed on six sections per animal, from three segments apart. Data (means ± SEM) represent the number of positive cells/mm². *p < 0.01 at least (Mann-Whitney).