Figure 5
Effect of MSC administration on MT mRNA expression and GST specific activity in spinal cord of SOD1/G93A mice. Animals were intravenously injected with 106 MSCs or saline at d 90. (A) Basal expression of MT-1, MT-2 and MT-3 mRNA in spinal cord from control SOD1 mice. MTs were analyzed by RT-PCR using GAPDH mRNA as a reference. Amplification curves of MT-1, MT-2 and MT-3 are shown with their crossing point on the xaxis. The difference between crossing points (ACt) are indicated. (B) Time course of the relative expression of MT-1, MT-2 and MT-3 mRNA. MTs were analyzed at d 30, 60 (presymptomatic mice), 90, 110 and 125 (symptomatic mice) by RT-PCR using GAPDH mRNA as a reference. Eight MSC-treated and seven saline-injected SOD1/G93A mice were used. Data are means ± SEM of two mRNA extractions from three experiments. (C) Expression of MT-1, MT-2 or MT-3 mRNA in MSC-injected SOD1/G93A mice at d 125. Data are reported as fold induction with respect to saline-injected SOD1/G93A mice and are means ± SEM of two mRNA extractions from three experiments. (D) Specific activity of GST in the spinal cord of saline-injected SOD1 and saline- or MSC-injected SOD1/G93A mice. GST activity was expressed as [xmol/min/mg protein. Data are means ± SEM of four experiments in duplicate. *p < 0.05 at least (ANOVA plus Bonferroni); **p < 0.001 (Mann-Whitney).