Figure 5
Binding of histone H3 to macrophages is diminished by the integrin αv&x#03B2;5 and Mer, but not by the αv&x#03B2;3 integrin. Chromeo 488-conjugated histone H3 (H3-488) (5 μg/mL) was incubated with BSA or Mer (A), integrin αv&x#03B2;3 (B) or integrin αv&x#03B2;5 (C) (5 μg/mL) for 30 min in RPMI-1640. The mixture of the proteins was then added to macrophages and incubated for 1 h, after which the cells were washed three times with PBS and the quantities of bound protein were determined by a fluorescent plate reader. ⋆⋆⋆p < 0.001 compared with the 488-H3 + BSA group.