Figure 6
Histone H3 inhibits efferocytosis in vivo.Mice (three in each group) were exposed to the intratracheal administration of 10 × 106 apoptotic neutrophils (A) or apoptotic thymocytes (B) resuspended in 50 μL PBS containing 5 μg BSA (control) or histone H3, and bronchoalveolar lavages were collected 2 h later. Cytospin slides were prepared from the bronchoalveolar lavage fluid, and phagocytosis was determined by microscopy (A) or by flow cytometry (B). ⋆⋆p < 0.05 compared with the BSA group. The percentage of macrophages that phagocytosed apoptotic cells for the control group is shown above the bar.