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Figure 2 | Molecular Medicine

Figure 2

From: Mammalian DNA Is an Endogenous Danger Signal That Stimulates Local Synthesis and Release of Complement Factor B

Figure 2

DNA stimulates factor B synthesis and release by macrophages in vitro. (A) Factor B mRNA expression in RAW264.7 macrophages treated for 6 h with Lipofectamine 2000 alone, calf thymus DNA (10 μg/mL) alone or calf thymus DNA (10 μg/mL) complexed with Lipofectamine 2000. *p < 0.001 versus control. (B) Immunohistochemistry for factor B and β-actin in RAW264.7 macrophages treated with DNA (10 μg/mL) complexed with Lipofectamine 2000, or Lipofectamine 2000 alone (control) for 6 h. (C) Factor B release into supernatants from RAW264.7 macrophages treated with DNA (10 μg/mL) complexed with Lipofectamine 2000 or Lipofectamine 2000 alone for 24 h. (D) Factor B protein expression in cell culture supernatants from RAW264.7 macrophages treated with DNA complexed with Lipofectamine 2000, or Lipofectamine 2000 alone for 24 h at various concentrations as indicated. (E) Expression of factor B mRNA in RAW264.7 macrophages treated for 6 h with mouse genomic DNA (10 μg/mL) complexed with Lipofectamine 2000. *p< 0.001 versus control. (F) Factor B mRNA expression in RAW264.7 macrophages treated for 6 h with poly (dAdT)poly(dTdA) (10 μg/mL) complexed with lipofection reagent. *p< 0.001 versus control. All experiments performed in triplicate, and repeated at least three times.

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