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Figure 6 | Molecular Medicine

Figure 6

From: Mammalian DNA Is an Endogenous Danger Signal That Stimulates Local Synthesis and Release of Complement Factor B

Figure 6

p38 and NF-κB mediate factor B production in macrophages. (A) Expression of factor B mRNA in RAW264.7 macrophages pretreated for 30 min with p38 inhibitor (SB 203580, 40 μmol/L) then treated with DNA (10 μg/mL) complexed with Lipofectamine 2000 for 6h. *p < 0.001 versus control, #p < 0.001 versus DNA + Lipofectamine 2000. (B) Factor B mRNA expression in RAW264.7 macrophages pretreated with NF-κB activation inhibitor (10

imol/L) overnight, then treated with DNA (10 μg/mL) complexed with Lipofectamine 2000 for 6 h. *p < 0.001 versus control, #p < 0.001 versus DNA + Lipofectamine 2000. Experiments were performed in triplicate and repeated at least three times.

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Molecular Medicine

ISSN: 1528-3658