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Figure 1 | Molecular Medicine

Figure 1

From: Inhibition of High-Mobility Group Box 1 Protein (HMGB1) Enhances Bacterial Clearance and Protects against Pseudomonas Aeruginosa Pneumonia in Cystic Fibrosis

Figure 1

Anti-HMGB1 mAb reduces Paeruginosa-induced pulmonary neutrophil recruitment and lung injury in CFTR-/- mice. Male CFTR-/- mice were treated with 250 µg/mouse of either anti-HMGB1 mAb (αHMGB1) or isotype control antibody (control) 12 h before and at the time of inoculation with GFP-expressing P. aeruginosa PAO1. Eighteen hours later, mice were euthanized, and lungs and BALs were harvested. Neutrophilic inflammation was assessed by the PMN in the BAL (A). Total protein content in BAL (B) and histological analysis (C) were used to assess lung injury. Mean ± SEM, *P < 0.05 (n = 5–6 mice per group of three independent experiments). (C) Representative images of HE-staining lung sections.

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