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Figure 5 | Molecular Medicine

Figure 5

From: Inhibition of High-Mobility Group Box 1 Protein (HMGB1) Enhances Bacterial Clearance and Protects against Pseudomonas Aeruginosa Pneumonia in Cystic Fibrosis

Figure 5

TLR4 mediates macrophage dysfunction in phagocytosis. Peritoneal macrophages were harvested from either C57BL/6 WT or mice deficient in TLR2 (TLR2-/-) or TLR4 (TLR4-/-). Macrophages were allowed to grow for 48 h and then treated with either normal human BAL or CF patient BAL and then exposed to heat-killed PAO1 for 1 h. Actin cytoskeleton was visualized by staining macrophages with phalloidin, and internalized PAO1 were counted and analyzed. Insets are images of peritoneal macrophages treated with normal healthy volunteer BAL (Norm). Scale bar, 10 µm. Histogram graphs represent mean ± SEM (n = 4). *P < 0.05 compared to macrophages treated with BAL samples of control subjects.

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Molecular Medicine

ISSN: 1528-3658