Figure 4
From: Neural Signaling in the Spleen Controls B-Cell Responses to Blood-Borne Antigen
The marginal zone cell arrest is neurally controlled. (A) The left branch of the vagus nerve was stimulated (VNS) 5 V, 2 ms, (5 Hz) for 2.5 min and mice were immunized immediately after. In sham mice the nerve was exposed but left untouched. Spleen sections obtained 15 min after immunization were stained with for CD11b and B220. (B) The number of follicles containing aggregated cells in VNS or sham-operated mice is shown. (C) Spleen sections from mice that underwent splenic neurectomy (SVNX) or from sham controls stained for CD11b, B220 and synaptophysin (top and center panels) or for CD11b, B220 and Gr-1 (lower panel) to show that CD11b+, but not Gr-1+ cells are absent from denervated areas. Images representative of 10 animals per group.