Figure 6
From: Neural Signaling in the Spleen Controls B-Cell Responses to Blood-Borne Antigen
Anatomical localization of innervated structures associated with ASCs. (A) Spleen sections from mice at 15 min after immunization were stained for MOMA-1, CD1d and B220. (B) Spleen sections from mice at 2 h after immunization were stained with a polyclonal anti-pan laminin antibody and CD138. (C) Spleen sections from mice euthanized 15 min after immunization stained for fibronectin, B220 and IgM. The asterisk indicates the area magnified in (E). (D) Higher magnification images (10X) reveal the clustering of CD138+ cells along laminin-containing structures detected as in b. (E) Staining was performed with a mouse monoclonal anti-synaptophysin antibody, which allowed the detection of both nerve fibers and antibody-expressing cells by utilizing a secondary FITC-conjugated anti-mouse IgM antibody (green). (F) Arrows denote IgM+ cells and arrowhead denote nerve fibers. Synaptophysin was detected with a polyclonal rabbit antibody. All images are representative of three independent experiments with at least 10 mice per group.