Figure 3

Confocal fluorescent immunohistochemistry for the nonphosphorylated neurofilament heavy-label SMI32 in retinal flat mounts from the experimental eye (GL) and respective fellow eye of representative untreated mice (NT) with 10 wks intraocular hypertension, mice receiving RHP before induction of 10 wks of intraocular hypertension, and sham controls. For each retina shown, superior regions are represented in panels A and B, E and F, I and J, M and N, and Q and R, and respective inferior regions are C and D, G and H, K and L, O and P and S and T. Temporal regions are A and C, F and H, I and K, N and P, and Q and S, and nasal regions are B and D, E and G, J and L, M and O, and R and T. Scale bar is 200 µm. The histogram in panel U shows the quantified results for SMI32 fluorescence intensity across all quadrants, in all animals, normalized to fellow eyes in each group. #P < 0.05 versus GL retina in the NT group.