Changes in body mass and metabolism. (A) Calming and exercise resulted in increased mean body mass (time*Calm, P < 0.0001; time*exercise, P < 0.0001). (B) Body mass of Calm mice was significantly (P < 0.05) greater after 7 d, compared with Cntl, and this difference grew over time. (C) Cntl Ex animals exhibited greater body mass than Cntl after 35 d (35 d, P < 0.05). (D) Calm Ex animals showed greater body mass after 7 d (P < 0.05) and this difference also increased. (E) Cntl and Calm animals had similar weekly energy intake, whereas Cntl Ex and Calm Ex animals displayed significantly greater energy intake, compared with Cntl. (F) MRI visualization of mouse lipid (red arrows). Cntl and Calm animals had similar amounts of lipid, whereas Cntl Ex and Calm Ex animals had less visible lipid. (G) Quantification confirmed that Cntl and Calm animals had similar adiposity, whereas Cntl Ex and Calm Ex animals were significantly leaner than Cntl (Cntl Ex, Calm Ex, P < 0.05). (H) Serum IGF-1 levels in Calm mice were lower than in Cntl (P < 0.05). (I) Serum insulin levels were higher in Calm and Calm Ex animals compared with Cntl (not significant). (J) Calm and Calm Ex animals displayed positive correlations between insulin and body mass and their slope differed significantly from Cntl (Calm, P < 0.001; Calm Ex, P < 0.05). Data represented as mean ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, ***P < 0.001.