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Figure 1 | Molecular Medicine

Figure 1

From: IL-1β Inhibits Human Osteoblast Migration

Figure 1

Influence of IL-1β on osteoblast migration under basal conditions (A) and toward C5a (B). In vitro migration was analyzed in a modified Boyden chamber using 8 µm polycarbonate filters. IL-1β was diluted in serum-free DMEM at 10 (light gray bars), 100 (dark gray bars) or 1000 (black bars) pg/mL and added either to both upper and lower or only to lower wells of the Boyden chamber as indicated. Serum-free DMEM was used as control (white bars). C5a was diluted in serum-free DMEM at 100 ng/mL. Columns represent mean number of totally migrated cells, bars represent SEM from three independent donors. Significance was calculated with two-way ANOVA followed by a Bonferroni post hoc test; *P <: 0.05. (A) Migration of osteoblasts with IL-1β in upper and lower (left) or only in lower wells (right). (B) Migration of osteoblasts toward a C5a gradient with IL-1β in upper and lower (left) or only in lower wells (right).

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