Figure 4

Influence of an IL-1β gradient on MSC migration toward PDGF-BB. In vitro migration was analyzed in a modified Boyden chamber using 8 µm polycarbonate filters. IL-1β was diluted in serum-free DMEM at 100 pg/mL and added to the lower wells of the Boyden chamber as indicated (black bars). Serum-free DMEM was used as control (white bars). PDGF-BB was diluted in serum-free DMEM at 10 ng/mL. Columns represent mean number of totally migrated cells, bars represent SEM from three independent donors.