Figure 7

Restoration of IL-1β-induced inhibition of osteoblast basal migration (A) and osteoblast migration toward PDGF-BB (B)—both through inhibition of ERK 1/2 and JNK signaling in osteoblasts. In vitro migration was analyzed in a modified Boyden chamber using 8-µm polycarbonate filters. IL-1β was diluted in serum-free DMEM at 100 pg/mL and added to the lower wells of the Boyden chamber as indicated (black bars). Serum-free DMEM was used as control (white bars). PDGF-BB was diluted in serum-free DMEM at 10 ng/mL. For inhibition of chemotaxis with MAPK inhibitors, 10 µmol/L PD 98059 (ERK1/2 inhibition) or JNK inhibitor II was added to upper and lower wells. Columns represent mean number of totally migrated cells, bars represent SEM from three independent donors. Significance was calculated with two-way ANOVA followed by a Bonferroni post hoc test; **P <: 0.01. Inhibition of ERK 1/2 and JNK signaling in osteoblasts in the presence of IL-1β (A) or both IL-1β and PDGFBB (B).