Spry-deficient murine lenses develop anterior subcapsular plaques. Representative histological sections of postnatal-d-80 WT mouse lenses (A) or mouse lenses deficient for all alleles of Spry1 and Spry2 (Spryfl/fl/Spry2fl/fl, B) using MLR10-Cre, stained with periodic acid Schiff reagent. The anterior monolayer of central lens epithelial cells (le) of WT mice (A) becomes multilayered in the absence of Spry (B), with cells closest to the restructured lens capsule (lc) appearing vacuolated. The more posterior cells acquire a spindle-shaped morphology as they deposit PAS-reactive ECM to form an anterior subcapsular plaque. Scale bar, 30 µm.