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Figure 6 | Molecular Medicine

Figure 6

From: Sprouty Is a Negative Regulator of Transforming Growth Factor β-Induced Epithelial-to-Mesenchymal Transition and Cataract

Figure 6

Epithelial cells from Spry-deficient murine lenses display TGFβ-like signaling. Representative sections of neonatal WT mouse lenses (A, A’, C, C’, E, E’) or lenses deficient for all alleles of Spry1 and Spry2 (Spryfl/fl/Spry2fl/fl) by using Le-Cre (B, B’, D, D’, F, F’), immunolabelled for either pSmad2 (A’, B’), Snail (C’, D’) or Snai2 (E’, F’), with cell nuclei counterstained with Hoechst dye (A–F). Compared to WT lens epithelia (le) that do not display nuclear reactivity for pSmad2 (A’), Snail (C’) or Snai2 (E’) lens cells deficient for Spry demonstrate a clear nuclear label for all of these downstream TGFβ-mediated signaling molecules (B’, D’, F’; arrows), prior to cell multilayering to form anterior subcapsular plaques. Scale bar, 50 µm.

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