Spry can prevent TGFβ-induced anterior subcapsular plaque formation. Representative histological sections of postnatal-d-10 (A) WT mouse lenses and transgenic mouse lenses (B) overexpressing TGFβ1 (TGFβ) or (C) both TGFβ1 and Spry1 (TGFβ/Spry1), stained withPAS reagent. The anterior monolayer of central lens epithelial cells (le) of WT mice (A) becomes multilayered with elevated levels of TGFβ1 (B), with elongating spindle-shaped cells reorganizing the overlying lens capsule (lc), as well as depositing PAS-reactive ECM (B, arrowhead) in the developing anterior subcapsular plaque. Increasing the levels of Spry1 specifically in lenses of transgenic mice overexpressing TGFβ1 prevents the formation of this anterior subcapsular plaque formation (C), with the lens epithelial cells remaining as a monolayer (C). Scale bar, 25 µm.