TH2 immune response instigates the loss of renal function and increases the fibrosis development. Pelvis proteinuria:creatininuria (A) and hydroxyproline:protein (B) ratios in IL-4, IL-12 KO and WT animals 7 d after UUO. Type-1 collagen mRNA (C) and deposition (D and E) at 7 d after surgery in IL-4, IL-12 KO and WT animals. The Sirius red is represented by the proportion of the staining area to the total area of the field. qPCR was normalized to HPRT, and the mean of the WT control mice was considered 1. Dashed line represents WT animals not submitted to UUO. *p < 0.05. Scale bar, 100 µm.