Skip to main content
Figure 1 | Molecular Medicine

Figure 1

From: Potential of the Trifunctional Bispecific Antibody Surek Depends on Dendritic Cells: Rationale for a New Approach of Tumor Immunotherapy

Figure 1

Activation and proliferation of T cells induced by Surek and/or DCs. T cells were enriched from spleens of naive mice (see Materials and Methods). All groups contained 1 × 105 B78-D14 cells, which were cocultured with 1 × 106T cells, with or without 20 µg/mL bsAb Surek and 5 × 105 BM-derived DCs as indicated, for up to 6 d. (A) Expression of CD69 on CD8+ and CD4+ T cells analyzed by FACS staining after 24 h of coculturing. (B) Expression of CD62L on CD8+ and CD4+ T cells analyzed after 24 h by FACS staining. (C, D) T-cell numbers analyzed by FACS staining and enumerated using counting beads at d 1, 4 and 6 of coculture. CD8+ T-cell numbers increased by d 6 in the presence of DCs and Surek. Means and standard deviations from three experiments are shown.

Back to article page