Figure 2

XN treatment inhibits PCa cell migration and invasion. Migration (A) and invasion (B) assays were performed in Boyden chambers with conditioned media from human fibroblasts, since chemoattractants reveal a significant reduction of migratory and invasive capacity of XN-treated DU145 and PC3 cells. Experiments were done in triplicate and repeated thrice. Means ± SD are shown (*P < 0.05, **P < 0.01, ***P < 0.001). Migration inhibition by XN was detected by the wound-healing assay (C): monolayers of DU145 and PC3 cells were scraped and treated with 10 µmol/L XN. Cell migration was evaluated by photographing the same fields of the wound surface under an inverted microscope (10x magnification) at time 0 and after 16 h. Representative results from one of three independent experiments are shown. C (within all panels), control; T0, time zero.