Figure 6

Oral administration of XN in TRAMP mice reduces UG tract weights and retards adenocarcinoma progression. (A) UG weight average of 20 TRAMP mice/group, treated with vehicle alone or XN. Means ± SD are shown (P = 0.046). (B) H&E staining of typical dorsolateral prostate from TRAMP mice: histological grades classified as normal, low- and high-grade PIN, WD carcinoma, PHY and PD carcinoma (40× magnification). (C) Representative fields of each section of each mouse (10 animals/group) were scored for incidence of each histological grade: all animals (control and XN-treated) presented PIN (low and high PINs were classified together) and PHY lesions (100% incidence), whereas WD and PD carcinoma incidence decreased in XN-fed mice (*P < 0.05). (D) The UG weight average from PD-bearing animals, normalized to total body weight, was significantly lower in XN-treated mice (P = 0.003), indicating a strongly reduced PD tumor growth in treated animals. (E) Vessel analyses from 10-µm-thick tissue slices of PD samples show a mature vasculature covering large areas of the tumor in control samples; this structure is partially lost in XN-treated mice where vessels with extravascular erythrocytes are evident (20× magnification). C (within panels A and E), control.