Figure 3

Reciprocal correlation between vaccine-induced immune response and Treg numbers. (A) Total number of spots before and after vaccination for each patient (IFN-γ ELISPOT). (B) Absolute number of CD4⁺CD25^highCD127^low/−Foxp3⁺CD39⁺ Tregs before and after vaccination for each patient (flow cytometry). (C) Fold-change of T-cell response (total spot number) versus fold-change of Treg number (absolute number of CD4+CD25^highCD127^low/−Foxp3+CD39+ Tregs). p = 0.243; correlation coefficient r = −0.365, Spearman rank correlation.