High Immune Response Rates and Decreased Frequencies of Regulatory T Cells in Metastatic Renal Cell Carcinoma Patients after Tumor Cell Vaccination

Table 2 Absolute numbers of peptide-reactive T cells in the periphery (per 150,000 cells pre-/postvaccination).

Patient	TYMS	IGF-BP3	RGS-5	VEGF	MMP7	CA IX	PRUNE	TIF1	NY-ESO	Pool C^a	Pool E^b	DTH
MR-1	51/41^c	43/32	^d-/16	45/85	14/43^e	−/24	nd/nd	22/nd	81/112	−/15	12/51	+
MR-2	12/45	64/183	49/135	22/124	48/162	−/65	−/66	50/82	24/173	19/107	39/157	++
MR-4	23/70	38/107	123/40	80/70	76/157	−/29	105/58	nd/73	40/63	66/71	99/97	+
MR-5	−/17	−/60	19/41	32/41	24/42	−/23	nd/nd	−/37	−/64	−/64	13/73	+
MR-6^f	12/27	30/48	49/18	−/−	−/−	−/−	−/−	−/−	−/−	−/−	−/11	−
MR-7^f	−/−	−/−	−/50	−/13	−/46	−/−	nd/nd	13/55	−/−	22/126	15/46	+
MR-9^f	−/−	−/−	−/−	−/−	−/28	−/−	−/−	−/−	−/12	15/31	39/37	(+)
MR-10	60/20	64/13	82/49	77/33	56/45	32/−	32/19	89/57	69/29	69/54	123/155	(+)
MR-11	−/39	99/37	−/43	51/nd	−/−	42/−	−/−	29/46	142/255	52/107	128/210	+
MR-13	28/46	71/80	40/62	61/32	111/17	−/−	−/−	40/101	80/32	41/74	157/125	++
MR-14	23/36	76/141	39/49	−/126	−/64	28/33	−/14	52/67	16/44	−/63	12/70	++
MR-15	11/−	−/28	−/15	−/59	15/25	−/−	−/25	4/53	14/31	−/14	−/67	+

DTH, delayed-type hypersensitivity reaction; nd, not determined (not enough cells).
^aPool C contains the peptides for ORMDL3, AHNAK-rel, BTG1, PIG10, LENG4, CP and PRAME.
^bPool E contains the peptides for TIF1, CLIC1, ELAC2 and TMP1.
^cThe values represent absolute numbers of spots for pre- and postvaccination (pre/post) responses. These values are given after subtraction of background responses (that is, without peptide or with irrelevant Bcr/Abl peptide). The highest postvaccination response is given irrespective of the time point postvaccination (that is, at 6, 14, 22 or 36 wks).
^dNo values mean no reactivity (≤10 spots above background, which corresponds to a frequency of ≤1 = 1.5 × 10⁴ IFN-γ-secreting peptide-specific T cells).
^eValues representing a greater than two-fold increase in reactivity against the peptides postversus prevaccination are indicated in bold. With the larger peptide pools, a reactivity of ≥40 spots above background was considered positive.
^fShort protocol of vaccination starting with the middle dose of 10⁷ vaccine cells. DTH reactivity: −, negative; (+), weak; +, positive; ++, positive (>2 cm redness and/or induration).

ISSN: 1528-3658