Figure 4

RPE morphology in differentiating human iPS cells. (A) MEF control (left) and differentiated pigmented cells (right). Monolayer cell domes are shown (arrowheads), suggesting apical-basal fluid transportation, an essential function of RPE. (B) Pigmented colonies transferred to feeder plates showing RPE fate. Arrowheads indicate different stages: early (blue), intermediate (red) and mature (green). (C) Colony with mixture of pigmented and nonpigmented hexagonal cells. (D) Colony with most hexagonal cells being pigmented. (E, F) iPS-derived RPE with native RPE morphological characteristics, including prominent melanin granules and perinuclear location of melanin granules. (G, H) Human autopsy RPE. Scale bar, 200 µm.