Figure 5

Morphology (A–F), expression (G–H) and functional analyses (I) of our iPS-derived-RPE. Arrowheads indicate apical microvilli (AMv) in iPS-RPE (A, B) and in human autopsy donor RPE (D, E) at 15,000× and 60,000×. Li, lipofusin granule. (C, F) Arrowheads indicate adherens junction-like structure between RPE cells (3,000×). (G) Quantitative RT-PCR of RPE65, BEST1 and MFRP expression in autopsy RP iPS-RPE, undifferentiated iPS and donor fibroblast. (H) Immunoblot analysis of RPE65 (65 kDa) and CRALBP (34 kDa) expression in autopsy RPE, iPS-RPE, undifferentiated iPS and donor fibroblast. Total protein (20 µg/lane). (I) Phagocytosis of POS in iPS-RPE. Bovine POS (green) and ZO1 (red). White arrowheads indicate POS inside RPE. Scale bar in A–C, 1 µm.