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Figure 4 | Molecular Medicine

Figure 4

From: Peptide Mimetic of the S100A4 Protein Modulates Peripheral Nerve Regeneration and Attenuates the Progression of Neuropathy in Myelin Protein P0 Null Mice

Figure 4

The H3 peptide increases neuronal branching in primary cultures of hippocampal and motor neurons. (A) Representative micrographs of hippocampal neurons grown on the laminin substratum in the absence of soluble ligands (Ctl) or in the presence of S100A4 or H3. (B-E: Hippocampal neurons, field 120 × 120 µm.) Effect of S100A4 (10 µmol/L) and the H3 peptide on average neurite length per cell (B), number of primary neurites per cell (C), number of branching points per neurite length (D) and ratio of longest neurite length to total neurite length per cell (E) are shown. (F-I: motoneurons.) (F) Representative micrographs of motoneurons double stained for GAP-43 and neurofilament-HT (NF), field 100 × 30 µm. (G-J) Effect of S100A4 (10 µmol/L) and the H3 peptide on average neurite length per cell (G), number of primary neurites per cell (H), number of branching points per neurite length (I) and ratio of longest neurite length to total neurite length per cell (J). (B-J) ANOVA versus untreated controls (Ctl); four independent experiments were performed.

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