Figure 8
HMGB1 impairs macrophage efferocytosis. Peritoneal macrophages were divided into groups of control, macrophagesHMGB1 and apoptotic cellsHMGB1 as indicated in the figure. Macrophages in macrophagesHMGB1 were treated with HMGB1 (1 µg/mL) for 30 min followed by cocultured with apoptotic thymocytes for 90 min. Macrophages in apoptotic cellsHMGB1 were treated with culture medium for 30 min followed by coculturing with apoptotic thymocytes that were pretreated with HMGB1 (1 µg/mL) for 30 min. Macrophages in control were treated with culture medium for 30 min followed by coculturing with apoptotic thymocytes that were not pretreated with HMGB1. After treatments, all macrophages were stained and processed for analysis with the flow cytometry as described in the Figure 2 legend. (A) Representative scatter plots in each group. (B) Representative histograms in each group. (C) Statistic analysis of FACS assay data. Values are mean ± SEM and represent average of findings from two independent experiments with triplicate samples in each group. *P < 0.05 versus the control group.