Figure 4
αHMGB1 attenuates pulmonary vascular remodeling in mice. Effect of chronic hypoxia (21 d) on vascular remodeling in C57Bl6/J mice treated with aHMGB1 antibody or control IgG (50 µg every other day, IP) during hypoxia (n = 6 per group). Untreated normoxic and hypoxic C57BL6/J mice served as additional controls. (A–C) Representative photomicrographs of SMA stained lung sections from hypoxic control mice, IgG-treated mice and aHMGB1-treated mice, respectively (scale bar = 100 µm). Morphometric analyses of the number of partially and fully muscularized arterioles (D, E) and vessel wall thickness in normoxic WT mice, hypoxic WT mice, and hypoxic WT mice treated with control IgG antibody or µHGMB1 (F) are shown. (G) Effect of HMGB1 (1,000 ng/mL) on PDGF (10 ng/mL)-stimulated HPASMC proliferation by using (3H)thymidine incorporation. Data represent the mean ± standard error of the mean (SEM) of three independent experiments. Analysis of variance: *P < 0.05. n.s., Not significant.