A20 protein levels in muscles of mdx mice treated with AAV8-tMCK-A20 compared with saline. (A) Total lysates from quadriceps and diaphragm muscles of 8-wk-old mdx mice treated with AAV8-tMCK-A20 or saline as neonates were analyzed for A20 protein levels by Western blotting. Results from three representative mice are shown. (B) Quantification of A20 protein levels normalized to GAPDH levels are shown. *p < 0.05. n = 9 for saline-treated mdx mice; n = 12 for AAV8-tMCK-A20-treated mdx mice.