Protein levels of RelB and muscle differentiation factors MyoD and Myf-5.
(A) Total lysates from quadriceps and diaphragm muscles of 8-wk-old mdx mice treated with AAV8-tMCK-A20 (AAV8-A20) or saline as neonates were analyzed for RelB, MyoD and Myf-5 protein levels by Western blotting. Results from three representative mice are shown.
(B) Quantification of RelB and Myf-5 protein levels normalized to GAPDH levels are shown. *p < 0.05. n = 8 for saline-treated mdx mice; n = 8 for AAV8-tMCK-A20-treated mdx mice.