Histological analysis of quadriceps muscle of AAV8-tMCK-A20- and saline-injected mdx mice. (A) Muscle sections of quadriceps from C57 mice and mdx mice treated with AAV8-tMCK-A20 or saline were stained using hematoxylin and eosin to visualize muscle morphology and location of nuclei. (B) Quantification of number of fibers with centrally placed nuclei in quadriceps of saline- or AAV8-tMCK-A20-treated mdx mice. *p < 0.05. n = 9 for saline-treated mdx mice; n = 12 for AAV8-tMCK-A20-treated mdx mice. Scale bar = 150 µm.