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Figure 1 | Molecular Medicine

Figure 1

From: Effect of Recombinant α1-Antitrypsin Fc-Fused (AAT-Fc) Protein on the Inhibition of Inflammatory Cytokine Production and Streptozotocin-Induced Diabetes

Figure 1

Purification of recombinant human AAT-Fc. AAT-Fc was purified by using protein A affinity chromatography. The eluted fractions were analyzed by silver staining and Western blotting under the reduced (with 2-mercaptoethanol [2-ME]) and nonreduced conditions. (A) The silver staining results indicated that the molecular size of the nonreduced AAT-Fc protein was approximately 160 kDa (dimer; major band), whereas that of the reduced AAT-Fc protein was 85 kDa (monomer). (B) Schematic representation of AAT-Fc in the dimeric form, connected by two disulfide bonds in the hinge region. Western blotting with anti-human IgG1 (C) and rabbit anti-human AAT antibodies (D) indicated the presence of AAT-Fc protein in the eluates. Representative data from one of five independent experiments are shown.

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